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| Sandagdorj, Narantsatsral; Goo, Youn-Kyoung; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Li, Yan; Cao, Shinuo; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Badgar, Battsetseg; Xuan, Xuenan. |
| Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally... |
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Palavras-chave: Babesia gibsoni; Diagnosis; Enzyme-linked immunosorbent assay (ELISA); Thrombospondin-related adhesive protein (TRAP). |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3115 |
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| Iguchi, Aiko; Soma, Takehisa; Suzuki, Hiroshi; Xuan, Xuenan. |
| In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals. |
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Palavras-chave: Allele-specific real-time PCR; Babesia gibsoni; Drug resistance; M121I variant population. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4421 |
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